ABSTRACT
Objective: To investigate the antihistamine-releasing effect of a peptide isolated from wasp venom of Vespa orientalis. Methods: This peptide was separated from crude venom by chromatography methods and mass spectrometry. Then various concentrations (2, 4, 8, 16, 32, 64, 128 and 256 μmol/L) of the peptide were incubated with mast cells and lactate dehydrogenase assay was performed. Results: No significant effect was observed in lactate dehydrogenase absorbance under 128 μmol/L concentration. This implied that the peptide did not cause cell death in mast cells and consequently, histamine release did not happen. Moreover, the results showed the IC
ABSTRACT
Background and Aims: there has been scant information concerning antihypertrophic effects of vitamin D specifically on its cellular and molecular mechanisms. Sirtuin 1 [SIRT1] is regarded as a key deacetylase enzyme in cardiomyocytes which applies potential cardioprotective effects by functional regulation of different proteins. This study aimed to evaluate the effects of 1, 25-dihydroxyvitamin D on the hypertrophic markers and cardiac level of SIRT1 mRNA in rats following the aortic banding
Material and Methods: in this study, male Wistar rats [170-220g] were used, which were divided into 4 groups: rats subjected to hypertrophy without treatment [H], rats pretreated with 1, 25 dihydroxyvitamin D3 [H+VD], rats received propyleneglycol as a vitamin solvent [H+P], and intact animals which were elected as the control group. Arterial blood pressure was directly measured by the carotid cannulation. Transcription level of target genes was measured by real time polymerase chain reaction technique
Results: in H+VD group, systolic blood pressure as well as heart weight-to-body weight ratio decreased significantly compared to the group H [P<0.01]. Moreover, regarding hypertrophy marker genes in H+VD group, both atrial natriuretic peptide mRNA [H+VD: 64.8+/-14% vs. H: 127+/-26%; P<0.05] and brain natriuretic peptide mRNA [H+VD: 25.6+/-6% vs. H: 84.2+/-12%; P<0.01] levels decreased significantly. SIRT1 mRNA level was increased by 56.8+/-14% in group H and by 42.6+/-12% in group H+VD which were significant in comparison to the control group [P<0.01 and P<0.05, respectively]. No significant difference was noted between H+VD and H groups
Conclusions: the results of the present study revealed that administration of 1, 25- dihydroxyvitamin D decreases myocardial hypertrophy markers in rats following the abdominal aortic banding. The pressure overload-induced hypertrophy accompanies with SIRT1 mRNA upregulation, though antihypertrophic effects of vitamin did not nparticipate in SIRT1 transcription level